染料法抗体型 qPCR Mix 数据报告
下载热度:1868 次更新日期:2020-04-23
1. 产品介绍
采用 SYBR Green I 嵌合荧光法进行实时荧光定量 PCR 的专用 2×浓度预混液。产品含有优化浓度HotStart Taq DNA Polymerase、SYBR Green I、dNTPs、Mg2+、反应缓冲液和稳定剂等成分。主要用于基因组 DNA 靶序列和 RNA 反转录后 cDNA 靶序列的检测。

2. 产品列表


3. 产品特点
抗体修饰的热启动酶,扩增效率更高,产量高。
灵敏度更高,适合低拷贝基因。
预变性时间短,反应速度快。
重复性好,跨度均一,产品性能稳定。
荧光强度高,易检测。
使用方便,性价比高,降低实验成本。
4. 实验数据
1) A301 标准曲线检测
以 Hela 细胞 cDNA 稀释按 10 倍浓度为梯度进行稀释,并以检测 CT 值构建标准曲线。可见使用A301 扩增效率高、标准曲线线性范围宽、重复性好。 
2) 各种典型模板
A301 进行各种典型模板测试,扩增灵敏度高、特异性好。
3) A301 复杂模板检测 
以 Hela 细胞为模板,检测 High-GC 基因(G+C=72%)扩增效果。可见对于 A301 检测复杂模板扩增不受影响,且扩增特异性好。
5. 客户案例
客户单位:兰州大学生科院 细胞所。
材料:马铃薯 RNA 逆转录的 cDNA,5 倍稀释液。
为了验证引物的特异性,使用 16 个样本检测熔解曲线。具体如下,扩增反应完成后,通过逐渐增加温度同时监测每一步的荧光信号来产生熔解曲线。结果显示 2 个引物均为单峰,特异性较好。 
扩增曲线如下:
6. 引用文献
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