逆转录试剂盒数据报告
下载热度:2151 次更新日期:2020-04-23
1. 产品介绍
StarScript II M-MLV 反转录酶通过基因工程改造,加强了 M-MLV 的酶活性、提高了 cDNA 第一链的合成延伸能力和产量、提高对高温的耐受性、同时提高了对样品中的残留干扰物的耐受能力。适合于基因全长 cDNA 的合成、qPCR 实验中 RNA 模板的快速反转录等,cDNA 产量和长链 cDNA 合成能力优异;同时,在 50℃拥有优良的表现,因此可适用于复杂二级结构的 RNA 的反转录。
2. 产品特点
卓越的延伸能力:最长可合成 15 kb 的 cDNA。
灵敏的检测范围:微量的 total RNA 模板,低丰度的目的基因也能完美反转录。
优良的耐热特性:采用较高的反应温度(如 50℃)时,可以克服复杂二级结构及模板量极低的 RNA 反转录难题,获得良好的反转录效果。
高效的扩增性能:与进口同类产品相比,具有相当或更优异的反转录效率。
超快的合成速度:合成的 cDNA 用于 qPCR 实验,RT 反应时间 15 min 即可满足,缩短了实验时间。

3. StarScript II cDNA 第一链合成产品列表 

重点产品推荐:StarScript II cDNA 第一链合成预混试剂(含去基因组)(Cat#A224) 
4. StarScript II cDNA 第一链合成试剂盒的产品特点对比
5. 实验数据
1) 逆转录产物不同扩增长度检测

使用 StarScript II M-MLV 反转录酶的产物为模板,可以成功扩增 300 bp-10 kb 片段。

2) 不同浓度起始模板量检测
以逆转录产物为模板,进行 qPCR 扩增,检测两个表达水平不同的基因。使用产品 GenStar A224,在 10 ng-1 μg 的 RNA 模板浓度下,扩增性能稳定、检测结果准确。 
3) 50℃反应检测
RNA 模板浓度依次为 2.5 ng、25 ng、50 ng、100 ng、200 ng、500 ng。扩增模板为人 28s ribosomal RNA 基因(GC 69.4% 复杂二级结构)。使用产品 GenStar A214,可对极少量的 RNA 模板进行良好的反转录反应。 
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